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The sample volume was 600 L, pH values were determined by internal indicator molecules optimized for NMR 19,20

For the maximum LDH release control group, 4 l of lysis solution (Promega, USA) was added into each well and incubated for 30 mins to initiate cell lysis prior to the next step

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If untreated, NASH progresses to liver cirrhosis, scaring of the liver, and hepatocellular carcinoma

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