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To further support the impact of CBS on replenishing GSH levels under cystine-restricted conditions, we expressed a codon-optimized CBS (CBS OPT ), which is resistant to the designed sgRNA (Fig
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Briefly, isolated crypts were centrifuged for 2 min at 68 g , resuspended in the appropriate volume of cold mouse colonic organoid growth medium (bioGenous, K2204-MC) and embedded in equal volume of Matrigel matrix (ABW, 082703) at 50250 crypts/20-30 L in a 48-well plate
Steroid hormone synthesis in mitochondria
which also supplies glutathione to the human body
