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Protein concentrations were determined using an enhanced BCA protein assay kit (Beyotime)
These drugs include semaglutide (Ozempic, Wegovy, Rybelsus), dulaglutide (Trulicity), liraglutide (Victoza, Saxenda), and tirzepatide (Mounjaro, Zepbound)though tirzepatide is actually a dual GIP/GLP-1 receptor agonist
Quantitative real-time PCR was performed using SYBR Green (SYBR Select Master Mix, Applied Biosystems) on a ViiA7 system (Applied Biosystems), and results were analyzed using the 2ddCt methods
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This is important because many patients with alcohol use disorder (AUD) abuse other substances and suffer from fatty liver and other metabolic abnormalities
