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The clarified supernatant fraction containing His6-AV-Ubiquitin-semapeptide was transferred to a beaker to load onto Ni-NTA resins while the pellet fraction containing cell debris was discarded

Step 4: Invert the vial so the needle tip is submerged in the solution

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Warnings Cautions and warnings: Consult a health-care practitioner prior to use if you are pregnant or breast-feeding

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