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GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium

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doi:10.1159/000371821 18Busam KJ, Capodieci P, Motzer R, Kiehn T, Phelan D, Halpern AC

Tsinghua University, National Center for Liver Cancer & Shanghai Eastern Hepatobiliary Surgery Hospital, Beijing