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Following the removal of the His-SUMO tag by ULP1, the eluted protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 GL column with an elution buffer containing 20 mM HEPES (pH 7.4) and 200 mM NaCl

Although progress has been made in the field of ferroptosis, further studies are needed to determine whether ferroptotic inhibitors can reverse testicular dysfunction and restore male fertility

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By far the most studied cysteine supplement is the cysteine prodrug N-Acetylcysteine (NAC)