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At the indicated time (1, 2, 4, 6 and 22 h) an aliquot of 10 L was removed from each experimental reaction and pipetting into 100 L of 1% TFA solution to quench the reaction ( t = 0 min was determined using the reaction without enzyme)

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In 1982, Stallone starred as Vietnam veteran John Rambo, a former Green Beret, in the action film First Blood , [26] an adaptation of the eponymous novel by David Morell, though the script was significantly altered by Stallone during the film's production, [57] which was both a critical and box-office success

Wilimowska J, Florek E, Piekoszewski W