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The gonads were then incubated with anti-digoxigenin antibody (Sigma, #11333089001) O/N at 4C, followed by three washes in PBST, then developed in AP substrate BCIP/NBT (Sigma, #B5655)

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B Vitamins Deficiency: specifically vitamin B-12 (Riboflavin) can cause cracked lips that have difficulty healing

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