Individual molecular species of CE were analyzed using multiple reaction monitoring transitions and quantified according to the reference standard and reported as mol/g liver tissue
A comprehensive panel of primary antibodies was used to detect various proteins: FOXO4, p53, Ser15-phospho-p53, p21 (cyclin-dependent kinase inhibitor 1A), p16 (cyclin-dependent kinase inhibitor 2A), 3-HSD, CYP11A1, CYP17A1 (cytochrome P450 family 17 subfamily A member 1), and several senescence-associated secretory phenotype (SASP) factors including IL-1, IL-1, IL-6, IL-10, TNF-, and TGF-
Practical tips: Rotate sites daily to avoid lipohypertrophy or localized irritation
Standard Range As most studies dictate, use 1mL to 5mL per vial, depending on the dose and concentration of the peptide required
Long-term or chronic toxicity data do not exist
Pop-Busui R, Sima A, Stevens M