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In-depth mechanistic studies reveal that DCA primarily activates the Takeda G protein-coupled receptor 5 (TGR5) on the surface of intestinal epithelial and immune cells, triggering the NF-B signaling pathway and inducing the release of pro-inflammatory cytokines such as IL-1, IL-6, and TNF-, while simultaneously inducing ROS bursts that lead to DNA damage (Figure 2A)
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