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It is not recommended to reconstitute to a concentration less than 100 g/mL in ddH 2 O

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Huppert et al

Generally, the recommended dosage per week starts at 600 to 1,200 mg per week

As the name suggests, this is a form of cell death caused by copper, in which copper ions directly bind to protein lipoylation components in the tricarboxylic acid circulation pathway, causing abnormal aggregation of lipoylated proteins and loss of iron-sulfur cluster protein, resulting in protein toxic stress responses and cell death (172)