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Cells were maintained in a humidified environment of 5% CO 2 at 37 C, cultured until an optical confluence of 75-100%, detached with 0.05% trypsin-EDTA 1 (25300-054, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA), at 37 C for approximately 5 min, and split to new plates according to the experimental procedures

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Within 6 months, I had lost 70 pounds, and by the time the trial was over, I had lost a total of 132 pounds

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Key tips: focus on form (quality over quantity), rest 1-2 days between strength sessions to recover, and progressively challenge yourself (if 5 lb weights become easy, move to 8 lb, etc.)

It will require embedding kidney testing into diabetes and hypertension workflows, and aligning reimbursement to reward prevention, not just downstream dialysis care