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By contrast, short-chain fatty acids (SCFAs), particularly butyrate, exert protective effects by engaging GPR41/43 and inhibiting histone deacetylases, thereby reinforcing epithelial barrier integrity and suppressing NF-B/TGF- pathways (49)

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When folate cycling is impaired by MTHFR insufficiency, the dihydrofolate THF step accumulates dihydrofolate, competing with the BH2 BH4 reduction step for DHFR activity and NADPH