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d ) HPLC analysis of intracellular levels of ZZ1 and its acidic metabolites
The brown-black solid product was obtained through silica gel column chromatography
Conflating them produces wildly inconsistent expectations
The animals were housed under controlled laboratory conditions (temperature: 2124 C
Digestion-based DNA methylation analysis Percentage DNA methylation was assessed at CpG sites across CpG islands in CYP2R1, CYP27A1, CYP27B1, CYP24A1, and VDR genes using Qiagen EpiTect II methylation enzyme kits and PCR assays at the concentrations and cycle conditions recommended by the manufacturer
Intracellular ROS production Intracellular reactive oxygen species (ROS) levels were measured in treated cells using the oxidant-sensitive probe 2,7dichlorofluorescein diacetate [H 2 DCFDA] 11
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