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Subsequently, unique gene hit counts were calculated using featureCounts from the Subread package v.1.5.2 by summarizing and reporting the hit counts based on the gene id feature in the annotation file 26
The sample size was determined based on the following rationale: for system consistency validation, ninety-six parallel samples ( n = 96) were analyzed to comprehensively cover all detection channels of a single 96-well plate, a number that far exceeds conventional requirements for microbiological method validation and aims to provide highly stable mean and relative standard deviation (RSD) values
(7) Goncharova ND, Vengerin AA, Khavinson VKh, Lapin BA
The molecular weight of benzyl alcohol (108.14 g/mol) is small enough that the preservative does not interfere with most analytical assays, including HPLC, mass spectrometry, and standard immunoassay readouts
