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Thus, SCO suppressed the expression of pro-inflammatory cytokines in macrophages and promoted their polarization towards M2 phenotype, suggesting that SCO may be effective in treating macrophage-mediated inflammatory diseases
NRF2, a superstar of ferroptosis
Next the cells were centrifuged and re-suspended in 1 mL RIP lysis buffer (150 mM KCl, 25 mM TrisHCl (pH 7.4), 5 mM EDTA, 0.5% NP-40, with 100 U/ml RNase inhibitor and protease inhibitor cocktail)
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