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Growth hormone deficiency in 'little' mice results in aberrant body composition, reduced insulin-like growth factor-I and insulin-like growth factor-binding protein-3 (IGFBP-3), but does not affect IGFBP-2, -1 or -4
Drawing to the wrong line, especially in poor lighting or without reading glasses, leads to significant dosing errors
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A 0.5 ml portion of each assay preparation was added to a well in a 24-well plate, and they were incubated at 37 C in 5% CO 2 for 1 h
From a methodological standpoint, BPC-157 experiments often involve titration across multiple concentrations, time-course sampling, and pairing with imaging or molecular assays
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