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Beads were washed five times with low- and high-salt lysis buffers, and bound proteins were eluted by boiling the beads in 1 SDS loading buffer for 15 min at 100 C, followed by immunoblotting analysis

Both medications can be used by people with various health conditions, but certain conditions require special consideration

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Enzyme-linked immunosorbent assay (ELISA) Concentrations of inflammation-related proteins in cell culture media were quantified using Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions