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A measure of 1 L of cDNA was added to a 19 L reaction mixture containing 1 Platinum SYBR Green qPCR Supermix-UDG, ROX dye, and forward and reverse primers (125500 nM), and amplified as previously described (Table 1) were extracted from previous publications from other laboratories ( 18s ) and cyclophilin A ( Ppia ) were used to normalize the amount of transcripts in individual animal samples (CT

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Flow cytometry To quantify cell proliferation, 300,000 cells were plated in 100 mm dishes and treated as specified

The current level considered sufficient for folate in plasma (i.e