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Storage Quick Reference Pro tip: Community practice described in protocols suggests starting with 1mL when doses are in the 100-250mcg range this keeps draw volumes larger and more measurable

Briefly, mice were sacrificed by cervical dislocation and livers were removed, washed in PBS at 37 C and then transferred to 5 mL of HBSS

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For example, obese diabetics are a special case and should not be given the full mortality and morbidity benefit for the weight-loss indication in addition to the diabetes indication

International Journal of Impotence Research, 2000

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