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Preparation of intestinal organoids from isolated mouse intestinal crypts (1) Resuspend the crypts in DMEM/Nutrient Mixture F-12, then use a pre-wetted micropipette tip to aspirate 10 L of sample and add it to a cell counting plate to count the number of crypts in the isolate
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The beneficial significance of the egr-1 gene implies the healing process (i.e., trans-activation of many cognate target genes in healing tissue, including growth factors and cytokines (Khachigian, 2006) or cholestatic liver injury (Kim et al., 2006
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