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coli Rosetta 2(DE3) cells

Forman et al., 2004)

In mammals, the GPxs family consists of eight members (GPx1-GPx8) identified so far

Together, these results indicate that decreased GSH/GSSG redox/antioxidant capacity and increased oxidative stress in the autism brain may have functional consequence in terms of a chronic inflammatory response, increased mitochondrial superoxide production, and oxidative protein and DNA damage

After transfer of 100 L of the respective cell suspensions into a black, clear-bottom 96-well plate (Nunc, Rochester, NY), 5 mM of the substances of interest: buffer, reduced (Sigma-Aldrich, CAS-70-18-8, GSH) or oxidized (Sigma-Aldrich, CAS-27025-41-8, GSSG) glutathione, cysteine or cystine, -mercapthoethanol or DTT were added

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