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Metabolites , 13 (4), 465

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Protein (50 g) was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a Precast PAGE Gel (412%, APPLYGEN, Beijing, China) and electroblotted onto nitrocellulose membranes

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(PMC) A separate metabolism-focused paper reviewing human trial history makes a similar point: early signals of weight loss were not consistently reproduced in later studies, particularly when intensive diet/exercise protocols were included