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Also, begin caloric deficit and exercise program

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Samples were run on either a SeQuant ZIC-pHILIC LC column, 5 m, 150 4.6 mm (MilliporeSigma, Burlington, MA) with a SeQuant ZIC-pHILIC guard column, 20 4.6 mm (MilliporeSigma, Burlington, MA) or an Atlantis Premier BEH Z-HILIC VanGuard FIT column, 2.5 m, 2.1 mm 150 mm (Waters, Milford, MA)

doi: 10.1038/s41586-019-1705-2 61 DollSFreitasFPShahRAldrovandiMda SilvaMCIngoldIet al

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IGF-1 LR3 contributes indirectly by preserving lean muscle mass during fat loss