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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

Kim TH, Jiang HH, Lim SM, Youn YS, Choi KY, Lee S, et al

Personal experience, it seems, points to modest, context-dependent effects, rather than dramatic changes

However, rapid weight loss itself can influence thyroid hormone requirements

It is also sometimes prescribed after weight loss surgery to reduce the accompanying risk of gallstone formation (Magouliotis 2017)

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