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Sci J (2018) 38:37589
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NC was measured at mid-neck height, between the mid-cervical spine and mid-anterior neck while sitting with a straight back, all to the nearest 0.1 cm
doi: 10.3390/ijms222212298
This 1:1 ratio prevents peptide precipitation (which occurs in overly concentrated solutions) while avoiding excessive dilution that wastes refrigerator space and increases contamination risk across multiple draws
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