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Am J Physiol Cell Physiol , 295:C849C868

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Enzyme expression was induced by the addition of 100 M isopropyl -d-1-thiogalactopyranoside, the cultures were incubated for another 3.5 h at 30 C, 140 rpm using a 5 cm shaking diameter, and the final OD 600 (optical density at 600 nm) was determined using a CO8000 cell density meter (WPA biowave)

I personally have two copies of the MTHFR mutation and am alive and well (with some interventions), while others I have seen have just one copy of a mutated gene, and a lot of symptoms

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Li J, Liu J, Zhou Z, Wu R, Chen X, Yu C, Stockwell BR, Kroemer G, Kang R, Tang D