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For RNA sequencing, total RNA was extracted from iPS cell-derived HLOs using an RNeasy Plus Mini kit (Qiagen) following the manufacturers protocol
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However, approximately 70% of hepatic blood supply comes from the intestine via the portal vein, allowing fungi or their metabolites to translocate across the gut barrier and potentially exacerbate liver disease 10,12
The intention is also to allow the peptide to come directly in contact with the gut lining
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