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Angle: 45 to the skin surface (or 90 if pinching a larger fat fold)
The triple receptor mechanism is new, but the biological pathways involved are well-understood
For derivatization, 10 L of methoxyamine hydrochloride in pyridine (20 mg/mL) was added to each sample and then shaken at 30 C for 90 min, followed by the addition of 91 L of a fatty acid methyl ester (FAMEs) and N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) mixture, which was shaken for 30 min at 37 C
Switching between such agents requires careful consideration
You may also experience side effects if you restart a high dose too quickly
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