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Invitrogen SuperScript IV VILO Master Mix (Thermo Fisher Scientific) was employed to convert 250 ng of total RNA into cDNA as per the manufacturers instructions, including prior genomic DNA removal with kit-supplied ezDNase
The SLC16A1 gene, that encodes MCT1, is expressed in multiple tissues
The working internal standards were also diluted from stock solutions in methanol with 0.1% formic acid
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J Neurochem 54: 823827
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