The stirring continued for an additional 12 h at 50 C, after which the mixture was introduced to 10 mL of deionized water while being sonicated
Apple embryonic axes (25 mg were homogenised in 50 mM K-phosphate buffer pH 7.5 containing 5 mM DTT, 5% (w/v) glycerol, 0.1% (w/v) triton X-100, 0,1% (v/v) protease inhibitor cocktail and 2% (w/v) PVPP on ice
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As she was resistant to conventional therapy (GH 17.5 ng/mL, IGF-1 947 ng/mL), we introduced PAS-LAR
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