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For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again
Panagaki T, Michael M, Hlscher C
You can read the full article at Serotonin (5-HT) is an important neurotransmitter associated with psychiatric disorders, but its precise role in behavior has been poorly understood due to a lack of methods to specifically target adult brain serotonin synthesis
It slows down how fast the stomach empties, making people feel full longer