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The next day, samples were washed in dig wash buffer (wash buffer with 0.02% digitonin) and incubated in dig wash buffer containing pA-MNase (1:1000 dilution) for 1.5 h at 4 C
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On the other hand, a more recent study using the complemented donor acceptor bioluminescence resonance energy transfer (CODA-RET) technique demonstrated a D 4.7 R heteromerization-dependent gain of function of the D 2 R ( CODA-RET is a BRET assay that allows the measurement of ligand-induced changes in the interaction between G protein-coupled receptors (GPCRs) forming specific homomers or heteromers with transducer proteins, including G proteins (58)
Vegans and vegetarians are particularly at risk for B12 deficiency because plant-based foods do not naturally contain vitamin B12
it's essential to provide the right nutritional support for her developing puppies
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