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Bacteriostatic Water is a sterile , non-pyrogenic water solution containing 0.9% (9 mL/L) benzyl alcohol as a bacteriostatic preservative

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For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method