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GST pull-down vs immunoprecipitation Preparing your glutathione beads Loading your GST-fusion protein onto the glutathione beads Adding the interacting proteins Eluting the GST-fusion and interacting proteins Materials needed for GST pull-down assays Preparing glutathione agarose beads Loading GST-fusion proteins onto glutathione beads GST pull-downs are assays used to investigate protein-protein interactions
Severe lows can cause seizure, loss of consciousness, or require emergency help
In the three-chamber test, MIA mice demonstrated a profound impairment in social interaction, as evidenced by a significantly lower sociability index score of 6.7 1.3, compared to 29.5 1.3 in control mice
Fluorescence ratios were normalized (control set to 0.1) and averaged (with error bars) and results shown as mean traces of the f 340nm / f 380nm ratio SEM (error bars in both directions) with n - values indicating the number of experiments per data point