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The final pellet was re-suspended in DMEM containing penicillin, streptomycin, 10% FBS, 1 g/ml insulin (Sigma), and 20 ng/ml epidermal growth factor (EGF, Sigma)

While calorie balance still matters, diet quality, protein intake, metabolic health, and consistency play a larger role in long-term success

Weight loss outcomes were also more pronounced in this trial