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They tried calculators and careful dilution, but the failure point usually occurred when complexity increased, such as during travel or when doses were missed, and then tiny measurement errors amplified into missed doses or accidental overdoses

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MS2 analysis consisted of quadrupole isolation (isolation window 0.7) of precursor ion followed by collision-induced dissociation (CID) in the ion trap (AGC 1.8E4, normalized collision energy 35%, maximum injection time 120 ms)

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12mg dose achieved 24.2% reductionhighest recorded for any obesity medication