After an additional three washings with PBS, positive signals were developed with an enhanced chemiluminescence kit [2]
By systematically investigating the specific targets of WMSP and molecular mechanisms from the whole-animal level down to the cellular level, our research aimed to provide a solid scientific foundation for its clinical efficacy and to contribute new insights and experimental data to the modernization of TCM compound research

Lyophilized powder (before reconstitution): Safe to freeze: Yes Extends shelf life: Yes, up to 36 months Must be in moisture-proof container Thaw completely before opening (prevents condensation) Reconstituted injectable solution: Safe to freeze: No, don't freeze Why avoid: Ice crystals can damage peptide structure Exception: Some users freeze individual doses in insulin syringes (controversial, potency may decrease) Topical serums: Safe to freeze: Generally no Why avoid: Emulsions can separate, preservatives may not protect when frozen, texture changes upon thawing Some simple water-based solutions may tolerate freezing If you must freeze reconstituted peptides: Use small single-dose aliquots Thaw slowly in refrigerator (not at room temp or microwave) Use immediately after thawing Never refreeze Accept some potency loss Storage by copper peptide product type Different copper peptide products have different optimal storage
doi:10.1097/01.CCM.0000114811.60629.B5
They were used for diabetes for more than a decade before being approved for weight loss
That is what makes it different from every weight loss medication that came before it