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It was found that addition of the caspase inhibitor failed to rescue ROS-induced cell death, suggesting that RSL3-induced cell death occured in a caspase-independent manner
Similarly, berberine shows anti-apoptotic effects by reducing the overexpression of caspase-3 and Bax/Bcl-2 (Sadeghnia et al., 2017)
The 2 -Ct analysis method was used to calculate the relative expression levels of each sample
While they are related, the specific effects of GSNO and GSSG within cells can be quite different due to their distinct chemical structures and reactivities