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Jejunal tissue was isolated, first cleaned with PBS, then immediately flushed and stored in cold biopsy preservation buffer (BIOPS) (pH 7.1 (5.77 mM Na 2+ ATP, 10 mM Ca-EGTA buffer, 0.1 M free calcium, 6.56 mM MgCl 2 6H 2 O, 20 mM taurine, 20 mM imidazole, 60 mM K-lactobionate, 15 mM phosphocreatine, 0.5 mM DTT, 50 mM MES)