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The second hexa-histadine tag of the protein was not utilized in these experiments, but is relevant and must be noted because Ni-NTA was used to purify the GST-GFP for several of the experiments being discussed

Simultaneously, they arent absorbing vitamin B12 well because of changes to their digestive tract

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10.1016/j.jad.2020.12.143 79 ChiangJ