Reductase enzymatic activity The reductase activities of the WT and mutant (K8A, C20A and K47A) proteins were measured spectrophotometrically by recording the increase in absorbance at 260 nm using a UV-VIS spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan) as described previously 37
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Previous observational studies have reported an inverse association between -carotene levels and several cancers, including breast, lung, liver, and pancreatic cancers (Shareck et al., 2017)
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