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Abstract A feeding trial for 8 weeks was performed to explore whether nutritional supplementation of l-carnitine may minimize the adverse effects of high-fat diet (HFD) on tilapia growth performance, antioxidant, immune parameters, inflammatory response, histopathology of liver, kidney, and intestine, as well as hepatic lipid metabolism aiming to reveal the mechanism and providing a shred of molecular evidence in Nile tilapia ( Oreochromis niloticous )

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