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Psychometric assessment was carried out before starting the current treatment course using the Hamilton Depressive Scale (HAMD-17) and the Hamilton Anxiety Scale (HARS), MMSE was used for cognitive performance assessment

A total of 90 animals were picked into M9, washed three times with M9 to remove bacterial debris and then snap-frozen in liquid nitrogen at a final volume of 30 L

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If you freeze it, consider pre-loading it into single-use amounts to avoid this