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Libraries were pooled and sequenced on a NovaSeq X sequencing system (Illumina)

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While mild, occasional sweating during tirzepatide treatment may not require immediate medical attention, certain patterns and accompanying symptoms warrant prompt evaluation by a healthcare provider: Call 911 or go to the emergency department if sweating occurs with: Signs of severe hypoglycemia: confusion, severe shakiness, difficulty speaking, loss of consciousness, or seizures (requiring assistance from others) Chest pain, shortness of breath, or irregular heartbeat Severe abdominal pain, particularly if radiating to the back (potential pancreatitis) Signs of severe dehydration: decreased urination, extreme thirst, dizziness, or fainting Allergic reaction symptoms: rash, hives, facial swelling, or difficulty breathing Contact your doctor within 2448 hours if you experience: Persistent or worsening night sweats that disrupt sleep Sweating accompanied by fever, fatigue, or weight loss that is unexpected or outside your treatment plan Sweating episodes that consistently occur after meals or at specific times New-onset sweating that began after starting tirzepatide or increasing the dose Sweating severe enough to require frequent clothing changes or that interferes with daily activities Your healthcare provider will likely assess blood glucose patterns, review concurrent medications, evaluate hydration status, and consider whether dose adjustment is appropriate

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From week 8 onward, the mice were divided into two groups: one group continued on the established HCD

Dose ladder: start at the low end for the first 15 days