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2016;11:e0165267
Opaka B, onierczuk M, Grabowska M
H., Stoll, C
Invest 124 , 42234226 (2014)
The perfusion buffer (113 mM NaCl, 4.7 mM KCl, 0.6 mM Na 2 HPO 4 2H 2 O, 0.6 mM KH 2 PO 4, 12 mM NaHCO 3 , 10 mM KHCO 3, 1.2 mM MgSO 4 7H 2 O, 10 mM HEPES, 30 mM taurine, 10 mM BDM, 10 mM glucose), digestion buffer (100 g/mL Liberase TM (Roche), 0.0125 mM CaCl 2 in perfusion buffer) and stop buffers (increasing calcium and BSA concentration, final concentration 1.5 mM CaCl 2 and 10% BSA) were adapted to achieve a maximum yield of rod-shaped cardiomyocytes
Liver mass was not different with housing temperature, independent of genotype, but fasting liver triglyceride levels were significantly lower in male cold-stressed mice, independent of genotype, compared to TN controls (Fig
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