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Not something Id ever do

Federici, L

We thank Christopher Koscher for his assistance with protein expression and purification and Florian Grebien for help with establishing the RPE-1 cell lines stably expressing p53 and p53 mutants

Challenges associated with the formation of recombinant protein inclusion bodies in Escherichia coli and strategies to address them for industrial applications

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However, it is most commonly used by those with experience in structured supplementation