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As many of these compounds aren't new discoveries, they were researched decades ago and shelved, sometimes for commercial reasons, sometimes simply because society wasn't ready for at-home injecting

To the 10.000 rpm supernatant containing microsomes and cytosol fractions, 0.2 volume of 0.1 M calcium chloride solution was added, and further centrifuged at 10,000 rpm for 10 minutes to precipitate the microsomes