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Of the 13 tested allozymes, eight exhibited minimal to no detectable sulfating activity (D46N, P54A, G57V, T58M, R132H, R259Q, G261V, and G261W), while the other five exhibited differential sulfating activity in comparison with the wild-type (P134L, D176N, R215H, S229T, and P330L)

After BPC-157 treatment at different time points, the level of cell growth was measured using MTT