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The glutathione can then be degraded by the cytoplasmic ChaC family of -glutamylamine cyclotransferases [ChaC1 (ChaC glutathione specific gamma-glutamylcyclotransferase 1) and ChaC2 (ChaC glutathione specific gamma-glutamylcyclotransferase 2)] generating 5-oxoproline and the dipeptide Cys-Gly
For immunoblotting, samples were resolved on 8%, 10%, or 12% polyacrylamide minigels (Bio-Rad) and transferred onto a PVDF membrane (GE Healthcare Life Sciences, PA) by wet or semi-dry transfer
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